cx43 rabbit polyclonal antibody  (Thermo Fisher)

Journal: Cell Genomics

Article Title: Single-cell multi-modal integrative analyses highlight functional dynamic gene regulatory networks directing human cardiac development

doi: 10.1016/j.xgen.2024.100680

Figure Lengend Snippet: Wnt-based 2D monolayer- and BMP/Activin A-based 3D embryoid body-derived hPSC-CMs display distinct functional differences (A) Schematic of 2D and 3D hPSC-CM differentiation systems. (B) Immunostaining (left) and quantification (right) of proliferating CMs (Ki-67+ and TNNT2 +, as represented by arrows) ( n = 15–18). (C) Immunostaining (left) and quantification (right) reveal rod-shaped morphology using N-cad as a marker for cell boundary ( n = 3, 20 hPSC-CMs/condition). (D) Immunostaining of N-cad for cell boundary and Cx43 for gap junctions (left) and quantification of antibody fluorescent intensity (right) based on dashed yellow line. (E) Spontaneous calcium transient traces (left) and boxplot showing cycle length (right) ( n = 3, 6–16 hPSC-CMs/condition). (F) Immunostaining (left) and quantification (right) reveal mitochondria distribution and intensity ( n = 3; 15 hPSC-CMs/condition). Inset shows high-magnification views of the regions outlined by white box. Yellow arrowheads point to regions of aggregated mitochondria, whereas white arrowheads point to regions of well-distributed mitochondria. Dashed line: nucleus (n). (G) Oxygen consumption rate (OCR) analysis ( n = 3; n = 4–6 technical replicates/condition). (H) Basal respiration rate ( n = 3; n = 4–6 technical replicates/condition). (I) Extracellular acidification rate (ECAR) analysis ( n = 3; n = 4–6 technical replicates/condition). (B, C, and E–I) Data were analyzed using a two-tailed Student’s t test. p < 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Data are represented as mean ± SEM. Scale bar: 10 μM. pmol, picomole; min, minutes; Anti+Rot, Antimycin A and rotenone; mpH, 1/1,000th pH unit. See also Figure S1 .

Article Snippet: Cells were incubated in N-cad Mouse Monoclonal Antibody (Invitrogen, 1:200, cat: MA1-91128), Cx43 Rabbit Polyclonal Antibody (Invitrogen, 1:500, cat: 71–0700, lot: WC324579), and/or Anti-Cardiac Troponin T Mouse Antibody (Abcam, 1:200, ab8295) antibodies in blocking buffer overnight at 4°C.

Techniques: Derivative Assay, Functional Assay, Immunostaining, Marker, Two Tailed Test

Journal: Cell Genomics

Article Title: Single-cell multi-modal integrative analyses highlight functional dynamic gene regulatory networks directing human cardiac development

doi: 10.1016/j.xgen.2024.100680

Figure Lengend Snippet:

Article Snippet: Cells were incubated in N-cad Mouse Monoclonal Antibody (Invitrogen, 1:200, cat: MA1-91128), Cx43 Rabbit Polyclonal Antibody (Invitrogen, 1:500, cat: 71–0700, lot: WC324579), and/or Anti-Cardiac Troponin T Mouse Antibody (Abcam, 1:200, ab8295) antibodies in blocking buffer overnight at 4°C.

Techniques: Recombinant, Virus, DNA Extraction, In Vitro, Sequencing, CRISPR, Knock-Out, RNA Expression, Plasmid Preparation, Synthesized, Software, Imaging